Sentences with auxotroph
aux·o·troph
A a - While the purpose of early mutant isolations was mostly focused on the identification of auxotrophs, many of these early strains have been subjected to heavy mutagenesis.
- A plasmid containing this 3.1 kb of wild-type genomic DNA was cotransfected with a trp1 plasmid into protoplasts isolated from an auxotrophic thn1 mutant strain.
- In addition to testing the generality of fungal survival factors, auxotrophic mutants allow several key aspects of fungal biology to be assessed in vivo.
- These mutations result in plants that are stunted, have dark green leaves and show defects in flower development and timing of flowering, but unlike GA auxotrophs these mutants are not rescued by GA application.
- Previous work on Drosophila de novo purine synthesis genes showed that 4 mg/ml RNA supplementation can rescue the phenotype of auxotrophic mutants fed a defined medium.
- The data indicate that the mutations complement with respect to the auxotrophic phenotype when grown on Sang's medium.
- However, we observed that a fraction of survivors become uracil auxotrophs, because of repair events associated with more complex chromosomal rearrangements.
- Previous experiments had indicated that leu2 auxotrophs had no selective advantage in rich medium.